4/8/2023 0 Comments Home pathological bl3Grade 8 DepEd Curriculum Guide in English.207508700 Philippine Red Cross Learn First Aid pdf.Bachelor in Secondary Education (ESP001).Financial Accounting And Reporting (AC108).Entrepreneurship In Tourism And Hospitality (THC1109).Disaster Readiness & Risk Reduction (DRRR 01).National Service Training Program (NSTP 1).Accountancy and Business Management (ABM 1-6).Fundamentals of Accountancy, Business, and Management (113).National Service Training Program (NSTP 1 ).Science, Technology and the Society (STS01).Bachelor of Science in Customs Administration (BSCA-IA).This is the first report to identify and sequence these bacteria isolated from Humulus lupulus cones. Both Pseudomonas fluorescens and Pseudomonas stutzeri are known plant-colonizing bacteria ( 11, 12). agglomerans has caused occupational disease in people working with hops ( 9, 10). Pantoea agglomerans B元 has been found in the rhizosphere and as an endophyte in plant tissues and is generally considered to be beneficial to plant growth however, P. The assembled bacterial genomes were annotated via the NCBI Prokaryotic Genome Annotation Pipeline (PGAP) ( 8). An ANI test compares the submitted genome sequence against the genomes of the type strains and proxytype strains that are already in GenBank ( 7). The microbes were identified using whole-genome BLAST, and the NCBI confirms the identity using average nucleotide identity (ANI). The draft genome assemblies of the three hop bacteria, Pantoea agglomerans B元, Pseudomonas stutzeri CM14, and Pseudomonas fluorescens BL, are described in Table 1. Default parameters were used for all software, unless otherwise specified. The retained contigs best matched to the expected target genera and have greater than 100× coverage. In each case, these low-coverage contigs were also identified as a different taxonomic group with BLAST. Contiguous sequences (contigs) were selectively removed from the assembly if the average read coverage was below 5×. Contaminant sequences (those that originated from a nontarget organism) were identified and removed from the assembly following the blobtoolkit pipeline ( 6). Assembly contiguity and completeness were assessed using QUAST v4.6.0 and BUSCO v3.0.0, respectively ( 4, 5). An initial assembly was constructed from the trimmed reads using SPAdes v3.11.0 ( 3). TruSeq adapters and low-quality bases were trimmed using Trimmomatic v0.36 under default settings ( 2). Raw sequencing data were demultiplexed using the Illumina bcl2fastq conversion software v1.8.4. Sequencing was completed on an Illumina HiSeq 2500 instrument for 518 cycles to produce 250-bp paired-end reads. WGS libraries were prepared following the Kapa Biosystems HyperPlus kit (KR1145 -v3.16). The whole-genome sequencing (WGS) of the bacterium was performed at the Hubbard Center for Genome Studies (University of New Hampshire, Durham, NH, USA). A single colony was selected and grown overnight in liquid lysogeny broth (LB), and genomic DNA was isolated using the Wizard SV genomic DNA purification system (Promega, Madison, WI). Pseudomonas fluorescens BL was isolated from another hop cone from the same New Hampshire location in August 2018 this cone was vortexed in PBS and plated onto violet-red glucose agar plates. The hop cone was placed in phosphate-buffered saline (PBS), vortexed, and plated onto nutrient agar plates. Pantoea agglomerans B元 and Pseudomonas stutzeri CM14 were isolated from a hop cone from a residential New Hampshire location in August 2017.
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